Otto von Jizzmark Posted April 12 Share Posted April 12 Hi @Buckster. So, this is what’s on today’s schedule in the lab: identification of some Bacillus isolates, where it's a pretty safe bet that at least one of them will turn out to be B. anthracis – that’s anthrax. Now as I’m sure you know, this phylogenetic group is a bit of a bugger when it comes to characterisation because they are all so closely related, which makes it very tricky to separate the ones that are harmless from the ones that are lethal. Using toxin genes encoded in the plasmid is problematic because it doesn’t detect non-plasmid containing strains, and of course the sequences for the two pathogenic plasmids can be detected in uncharacterized genomes. There are some chromosomal regions that can be examined, but then again using real-time PCR to target the rpoB gene can throw up false positives among closely related non-anthrax species. So, we have various options, one of which is to use suppressive subtractive hybridisation to identify specific chromosomal sequences unique to B. anthracis and then multiplex PCR four separate loci. If we get a positive, we then have to go through the whole palaver of deactivation, decontamination and disposal, plus all the compliance paperwork that goes along with that. It’s all a bit of a faff and bloody expensive – you wouldn’t believe what we spend just on PCR arrays every month! So, I was wondering if a different approach might work. I remembered that those brainboxes over at one of those creationist websites where you get your proper science from wrote an article some years ago concluding that the real reason bacteria like B. anthracis are lethal when their closest relatives are not was because some of them turned bad when Adam ate the apple and allowed evil into the world. That got me thinking: do you suppose some sort of exorcism might be just as effective as what we’re doing? We could forego all the hassle and expense of identification and safe disposal and just assume they were all naughty, get the local vicar in to sprinkle some holy water and mumble some Latin over them and then just chuck the lot straight in the bin. Obviously, if it works I’ll happily pay you a consideration for your consultancy services commensurate with the savings we make in efficiency and the reduction in our consumables expenditure. What do you reckon? All thoughts greatly appreciated… 1 Link to comment Share on other sites More sharing options...
XTreme Posted April 12 Share Posted April 12 This sort of intellectual shit requires someone with the mental capacity of @Marcel le Moose Fondler to advise! 5 Link to comment Share on other sites More sharing options...
Saul Posted April 12 Share Posted April 12 22 minutes ago, XTreme said: This sort of intellectual shit requires someone with the mental capacity of @Marcel le Moose Fondler to advise! He is too busy trying to brew some homemade Rohypnol for his summertime plans. 4 Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 36 minutes ago, XTreme said: This sort of intellectual shit requires someone with the mental capacity of @Marcel le Moose Fondler to advise! Has a great brain said many years ago...science without religion is lame...and religion without science is blind... Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 So from what I could read fast ..we're gonna dis- credit Buckster idea of young earth thing with micro biology...working on one of those German cars this morning..wich I hate doing...give me few to download info on the micro world ...not a subject I'm very familiar with... Link to comment Share on other sites More sharing options...
Otto von Jizzmark Posted April 12 Author Share Posted April 12 9 minutes ago, Marcel le Moose Fondler said: So from what I could read fast ..we're gonna dis- credit Buckster idea of young earth thing with micro biology...working on one of those German cars this morning..wich I hate doing...give me few to download info on the micro world ...not a subject I'm very familiar with... No, not at all. I'm just wondering if this might save me some time and money. 1 Link to comment Share on other sites More sharing options...
Otto von Jizzmark Posted April 12 Author Share Posted April 12 And if Buckster isn't sure then I've got an experiment in mind that you're going to love. Of course, the local padre might not enjoy it quite so much if it doesn't work... 4 Link to comment Share on other sites More sharing options...
XTreme Posted April 12 Share Posted April 12 1 minute ago, Otto von Jizzmark said: And if Buckster isn't sure then I've got an experiment in mind that you're going to love. Of course, the local padre might not enjoy it quite so much if it doesn't work... Somebody trolling @Buckster! Never thought I'd see the day! 1 Link to comment Share on other sites More sharing options...
XTreme Posted April 12 Share Posted April 12 And @Otto von Jizzmark did you want to switch to the Professor Wankstain username? Link to comment Share on other sites More sharing options...
Otto von Jizzmark Posted April 12 Author Share Posted April 12 No! It's bad enough that I spent five minutes the other day trying to log in as Otto von Wankstain thanks to Buckster! 4 Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 11 minutes ago, Otto von Jizzmark said: No, not at all. I'm just wondering if this might save me some time and money. Well if it's money you want to save on car repairs...I'm your man...lol. and I prefer cash only... 1 Link to comment Share on other sites More sharing options...
Buckster Posted April 12 Share Posted April 12 I appreciate that you are trying to add some value to your pseudo science however you misunderstand the nature of sin. An exorcism is only useful in the case if a demon possessed person. Sin alas cannot be fully mortified in our fallen world and will be with us until Christ returns. The best I can offer you are the services of @Marcel le Moose Fondler and his extremely effective anthrax testing procedure, also known as "the lick test". 1 2 Link to comment Share on other sites More sharing options...
Catteeclan Posted April 12 Share Posted April 12 1 hour ago, Marcel le Moose Fondler said: So from what I could read fast ..we're gonna dis- credit Buckster idea of young earth thing with micro biology...working on one of those German cars this morning..wich I hate doing...give me few to download info on the micro world ...not a subject I'm very familiar with... German car indeed. 1 4 Link to comment Share on other sites More sharing options...
XTreme Posted April 12 Share Posted April 12 5 minutes ago, Catteeclan said: German car indeed. I thought Volvo were Swedish? 2 Link to comment Share on other sites More sharing options...
Buckster Posted April 12 Share Posted April 12 6 minutes ago, XTreme said: I thought Volvo were Swedish? Link to comment Share on other sites More sharing options...
Catteeclan Posted April 12 Share Posted April 12 6 minutes ago, XTreme said: I thought Volvo were Swedish? and now owned by china. 3 Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 9 minutes ago, Catteeclan said: and now owned by china. Anything not american made or Japanese...I classifying them has German.. 1 2 Link to comment Share on other sites More sharing options...
Buckster Posted April 12 Share Posted April 12 16 minutes ago, Catteeclan said: and now owned by china. I thought Ford owned Volvo. Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 Just now, Buckster said: I thought Ford owned Volvo. Are you insuavating that the professor is posses by the devil ? Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 2 minutes ago, Buckster said: I thought Ford owned Volvo. I hate ford's also....frigen crap... Link to comment Share on other sites More sharing options...
XTreme Posted April 12 Share Posted April 12 33 minutes ago, Marcel le Moose Fondler said: Anything not american made or Japanese...I classifying them has German.. Doesn't that make ordering parts a bit difficult? Link to comment Share on other sites More sharing options...
Marcel le Moose Fondler Posted April 12 Share Posted April 12 15 minutes ago, XTreme said: Doesn't that make ordering parts a bit difficult? Yes more difficult to find parts for those exotic brands... 1 Link to comment Share on other sites More sharing options...
Catteeclan Posted April 12 Share Posted April 12 1 hour ago, Buckster said: I thought Ford owned Volvo. They did. 1 Link to comment Share on other sites More sharing options...
Otto von Jizzmark Posted April 12 Author Share Posted April 12 2 hours ago, Buckster said: I appreciate that you are trying to add some value to your pseudo science however you misunderstand the nature of sin. An exorcism is only useful in the case if a demon possessed person. Sin alas cannot be fully mortified in our fallen world and will be with us until Christ returns. The best I can offer you are the services of @Marcel le Moose Fondler and his extremely effective anthrax testing procedure, also known as "the lick test". This is what I was afraid of. None of my good ideas ever turn out the way I expected. That Dyson Ball Vac I bought was a massive disappointment as well - awkward to use and no better than washing them in the shower. And as for the ultrasonic ring cleaner... 1 4 Link to comment Share on other sites More sharing options...
Sir Fallsalot Posted April 12 Share Posted April 12 5 hours ago, Otto von Jizzmark said: Hi @Buckster. So, this is what’s on today’s schedule in the lab: identification of some Bacillus isolates, where it's a pretty safe bet that at least one of them will turn out to be B. anthracis – that’s anthrax. Now as I’m sure you know, this phylogenetic group is a bit of a bugger when it comes to characterisation because they are all so closely related, which makes it very tricky to separate the ones that are harmless from the ones that are lethal. Using toxin genes encoded in the plasmid is problematic because it doesn’t detect non-plasmid containing strains, and of course the sequences for the two pathogenic plasmids can be detected in uncharacterized genomes. There are some chromosomal regions that can be examined, but then again using real-time PCR to target the rpoB gene can throw up false positives among closely related non-anthrax species. So, we have various options, one of which is to use suppressive subtractive hybridisation to identify specific chromosomal sequences unique to B. anthracis and then multiplex PCR four separate loci. If we get a positive, we then have to go through the whole palaver of deactivation, decontamination and disposal, plus all the compliance paperwork that goes along with that. It’s all a bit of a faff and bloody expensive – you wouldn’t believe what we spend just on PCR arrays every month! So, I was wondering if a different approach might work. I remembered that those brainboxes over at one of those creationist websites where you get your proper science from wrote an article some years ago concluding that the real reason bacteria like B. anthracis are lethal when their closest relatives are not was because some of them turned bad when Adam ate the apple and allowed evil into the world. That got me thinking: do you suppose some sort of exorcism might be just as effective as what we’re doing? We could forego all the hassle and expense of identification and safe disposal and just assume they were all naughty, get the local vicar in to sprinkle some holy water and mumble some Latin over them and then just chuck the lot straight in the bin. Obviously, if it works I’ll happily pay you a consideration for your consultancy services commensurate with the savings we make in efficiency and the reduction in our consumables expenditure. What do you reckon? All thoughts greatly appreciated… And now i know how Pete feels when i talk about tools or DIY, what does the red one taste like must be nice because there's only one left 5 Link to comment Share on other sites More sharing options...
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